Réseau secret - An Overview
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When demultiplexing, the ultimate sample sheet offered for the FASTQ generation program ought to provide the i5 index in the correct orientation for that System. On the other hand, most Illumina sample sheet era software program handles this automatically.
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Index 2: the i5 index column is during the reverse enhance orientation (would be the reverse complement of your i5 indexes within the sample sheet).
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For operates uploaded manually (i.e., an Illumina Device is just not being used to crank out the sample sheet), the index orientation is dependent upon which instrument you might be using. You should see the Index Orientation Desk for information on tips on how to enter the i5 sequence with regards to the instrument and software program made use of.